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vivo imaging system  (Revvity)


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    Structured Review

    Revvity vivo imaging system
    Vivo Imaging System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 37442 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vivo+imaging+system/IVIS+optical+imaging+platform/pmc13011233-533-11-15
    Average 96 stars, based on 37442 article reviews
    vivo imaging system - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Biomimetic injectable engineered hierarchical porous microspheres for enhanced synergistic cell therapy of critical limb ischemia
    Article Snippet: .. Fluorescence intensity changes over 21 days were monitored using an in vivo imaging system (IVIS, PerkinElmer IVIS Lumina II, USA). ..

    Article Title: Intranasally delivered fucoidan nanozyme–decorated photosynthetic Chlamydomonas regulates vascular pathology and hypoxia in diabetes-associated retinal microvascular dysfunction
    Article Snippet: .. To investigate the biodistribution and metabolic dynamics of IND CHL@Pro-Fu-PEDOT-NZs in a DR mouse model, we employed an in vivo imaging system (IVIS (PerkinElmer IVIS Lumina III XRMS)) to monitor the spatial distribution of Chlorophyll-derivative red fluorescence across various tissues. ..

    Article Title: Injectable hydrogel vaccine enhances lymph node immunity for cancer immunotherapy
    Article Snippet: Injectable hydrogel platforms have emerged as a powerful localized approach to direct and potentiate immune responses within draining lymph nodes (dLNs), the critical orchestrators of systemic anti-tumor immunity.. To address the challenge of efficiently targeting delivery to dLNs, we developed an integrated nanovaccine platform, designated HM@FP Gel, by co-encapsulating hybrid membrane nanovesicles, co-delivery of autonomous tumor antigen and adjuvant signals, with a bifunctional anti-PD-1/IL-2 fusion protein within a thermosensitive, photocrosslinkable collagen matrix.. Upon subcutaneous injection, this platform forms an in-situ depot that provides sustained co-delivery, achieving ~ 80% cumulative release of both payloads over 4 days in vitro and significantly prolonging local retention while enhancing targeted accumulation in dLNs compared to free formulations.

    Article Title: Multifunctional sericin/alginate hydrogel integrated with cerium oxide nanozyme for accelerating methicillin-resistant Staphylococcus aureus-infected wound healing
    Article Snippet: Wounds complicated by multidrug-resistant (MDR) bacterial infections have posed a severe clinical challenge.. The development of innovative and efficient antibacterial materials is of great importance for the management and treatment of MDR-infected wounds.. Herein, an injectable sericin/alginate-based hydrogel (CeBG) was constructed by the incorporation of antibacterial compound berberine (BBR)-containing cerium oxide nanozyme.

    In Vivo Imaging:

    Article Title: Biomimetic injectable engineered hierarchical porous microspheres for enhanced synergistic cell therapy of critical limb ischemia
    Article Snippet: .. Fluorescence intensity changes over 21 days were monitored using an in vivo imaging system (IVIS, PerkinElmer IVIS Lumina II, USA). ..

    Article Title: Intranasally delivered fucoidan nanozyme–decorated photosynthetic Chlamydomonas regulates vascular pathology and hypoxia in diabetes-associated retinal microvascular dysfunction
    Article Snippet: .. To investigate the biodistribution and metabolic dynamics of IND CHL@Pro-Fu-PEDOT-NZs in a DR mouse model, we employed an in vivo imaging system (IVIS (PerkinElmer IVIS Lumina III XRMS)) to monitor the spatial distribution of Chlorophyll-derivative red fluorescence across various tissues. ..

    Article Title: Doping-Engineered Proangiogenic Nanozymes Orchestrate Ischemic Tissue Regeneration via Cytoprotection and Revascularization
    Article Snippet: .. At set time points, the mice were anesthetized with isoflurane, and the fluorescent signal in the legs was recorded using an in vivo imaging system (IVIS Lumina III, PerkinElmer, USA). ..

    Article Title: Oral Poly(N-oxide) zwitterionic nanoplatform for Gambogenic Acid Enhances Mucosal penetration for potentiated anti-angiogenic therapy
    Article Snippet: .. To monitor the orthotopic tumor growth, each mouse was anesthetized with isoflurane and executed intraperitoneal injection with 150 mg/kg D-Luciferin potassium solution (0.1 mL), then the bioluminescence of orthotopic hepatic tumors were measured by in vivo imaging system (PerkinElmer IVIS spectrum) in 5 min. ..

    Article Title: ASCL2 drives colorectal cancer metastasis through transcriptional activation of TDGF1.
    Article Snippet: .. The growth of metastatic foci was detected using an in vivo imaging system (IVIS Lumina XRMS Series III; PerkinElmer, USA). .. The mice received an intraperitoneal injection of 150 mg/kg D-luciferin potassium (HY-12591B; MedChemExpress, USA) and were then anesthetized with isoflurane 10 min after injection, after which whole-body images were acquired using a bioluminescence imaging system.

    Article Title: Injectable hydrogel vaccine enhances lymph node immunity for cancer immunotherapy
    Article Snippet: Injectable hydrogel platforms have emerged as a powerful localized approach to direct and potentiate immune responses within draining lymph nodes (dLNs), the critical orchestrators of systemic anti-tumor immunity.. To address the challenge of efficiently targeting delivery to dLNs, we developed an integrated nanovaccine platform, designated HM@FP Gel, by co-encapsulating hybrid membrane nanovesicles, co-delivery of autonomous tumor antigen and adjuvant signals, with a bifunctional anti-PD-1/IL-2 fusion protein within a thermosensitive, photocrosslinkable collagen matrix.. Upon subcutaneous injection, this platform forms an in-situ depot that provides sustained co-delivery, achieving ~ 80% cumulative release of both payloads over 4 days in vitro and significantly prolonging local retention while enhancing targeted accumulation in dLNs compared to free formulations.

    Article Title: Multifunctional sericin/alginate hydrogel integrated with cerium oxide nanozyme for accelerating methicillin-resistant Staphylococcus aureus-infected wound healing
    Article Snippet: Wounds complicated by multidrug-resistant (MDR) bacterial infections have posed a severe clinical challenge.. The development of innovative and efficient antibacterial materials is of great importance for the management and treatment of MDR-infected wounds.. Herein, an injectable sericin/alginate-based hydrogel (CeBG) was constructed by the incorporation of antibacterial compound berberine (BBR)-containing cerium oxide nanozyme.

    Article Title: Sphingosine‐1‐Phosphate Receptor 3 Confers Tumor Metastasis in Lung Cancer Resistant to Third‐Generation EGFR Inhibitor
    Article Snippet: .. An in vivo imaging system (IVIS, PerkinElmer) was used to monitor metastasis weekly. ..

    Injection:

    Article Title: Oral Poly(N-oxide) zwitterionic nanoplatform for Gambogenic Acid Enhances Mucosal penetration for potentiated anti-angiogenic therapy
    Article Snippet: .. To monitor the orthotopic tumor growth, each mouse was anesthetized with isoflurane and executed intraperitoneal injection with 150 mg/kg D-Luciferin potassium solution (0.1 mL), then the bioluminescence of orthotopic hepatic tumors were measured by in vivo imaging system (PerkinElmer IVIS spectrum) in 5 min. ..



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    Design and validation of bone-targeted Bmp2. a. Schematic representation of the Bmp2/D-Bmp2 plasmid constructs and the structure predicted by AlphaFold3. b. Immunofluorescence staining of HEK-293T cells transfected with the plasmids: phalloidin (green), DAPI (blue), and Alexa Fluor 647-conjugated anti-Flag antibodies (red). Scale bar: 10 μm. c. SDS-PAGE of purified proteins. d. Western blot validation of protein expression. e. Bmp2 activity reporter assay: schematic of the luciferase reporter system (left), representative fluorescence images, and statistical analysis of firefly luciferase activity by in vivo imaging system <t>(IVIS)</t> (a.u.: arbitrary units) (right, n = 3 per group). f. qPCR analysis of Bmp2 signaling pathway-related mRNA levels in MC3T3-E1 cells treated with the Bmp2 or D-Bmp2 protein (n = 3 per group). g, h. Western blot (g) and quantification of Bmp2 pathway-related protein expression in treated MC3T3-E1 cells (h) (n = 3 per group). i. Schematic of the HA-coated ELISA plate and HA-binding affinity assay (n = 3 per group). j. Tissue-specific binding assay of Bmp2 or D-Bmp2: Mouse muscle slices (left, scale bar: 100 μm) and undecalcified femur slices (right, scale bar: 200 μm) stained with AF647-conjugated anti-Flag antibodies. The data are presented as the means ± standard deviations (SDs). One-way ANOVA was used for multiple comparisons. Significance levels: ns (not significant), ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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    Design and validation of bone-targeted Bmp2. a. Schematic representation of the Bmp2/D-Bmp2 plasmid constructs and the structure predicted by AlphaFold3. b. Immunofluorescence staining of HEK-293T cells transfected with the plasmids: phalloidin (green), DAPI (blue), and Alexa Fluor 647-conjugated anti-Flag antibodies (red). Scale bar: 10 μm. c. SDS-PAGE of purified proteins. d. Western blot validation of protein expression. e. Bmp2 activity reporter assay: schematic of the luciferase reporter system (left), representative fluorescence images, and statistical analysis of firefly luciferase activity by in vivo imaging system <t>(IVIS)</t> (a.u.: arbitrary units) (right, n = 3 per group). f. qPCR analysis of Bmp2 signaling pathway-related mRNA levels in MC3T3-E1 cells treated with the Bmp2 or D-Bmp2 protein (n = 3 per group). g, h. Western blot (g) and quantification of Bmp2 pathway-related protein expression in treated MC3T3-E1 cells (h) (n = 3 per group). i. Schematic of the HA-coated ELISA plate and HA-binding affinity assay (n = 3 per group). j. Tissue-specific binding assay of Bmp2 or D-Bmp2: Mouse muscle slices (left, scale bar: 100 μm) and undecalcified femur slices (right, scale bar: 200 μm) stained with AF647-conjugated anti-Flag antibodies. The data are presented as the means ± standard deviations (SDs). One-way ANOVA was used for multiple comparisons. Significance levels: ns (not significant), ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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    Design and validation of bone-targeted Bmp2. a. Schematic representation of the Bmp2/D-Bmp2 plasmid constructs and the structure predicted by AlphaFold3. b. Immunofluorescence staining of HEK-293T cells transfected with the plasmids: phalloidin (green), DAPI (blue), and Alexa Fluor 647-conjugated anti-Flag antibodies (red). Scale bar: 10 μm. c. SDS-PAGE of purified proteins. d. Western blot validation of protein expression. e. Bmp2 activity reporter assay: schematic of the luciferase reporter system (left), representative fluorescence images, and statistical analysis of firefly luciferase activity by in vivo imaging system <t>(IVIS)</t> (a.u.: arbitrary units) (right, n = 3 per group). f. qPCR analysis of Bmp2 signaling pathway-related mRNA levels in MC3T3-E1 cells treated with the Bmp2 or D-Bmp2 protein (n = 3 per group). g, h. Western blot (g) and quantification of Bmp2 pathway-related protein expression in treated MC3T3-E1 cells (h) (n = 3 per group). i. Schematic of the HA-coated ELISA plate and HA-binding affinity assay (n = 3 per group). j. Tissue-specific binding assay of Bmp2 or D-Bmp2: Mouse muscle slices (left, scale bar: 100 μm) and undecalcified femur slices (right, scale bar: 200 μm) stained with AF647-conjugated anti-Flag antibodies. The data are presented as the means ± standard deviations (SDs). One-way ANOVA was used for multiple comparisons. Significance levels: ns (not significant), ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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    Design and validation of bone-targeted Bmp2. a. Schematic representation of the Bmp2/D-Bmp2 plasmid constructs and the structure predicted by AlphaFold3. b. Immunofluorescence staining of HEK-293T cells transfected with the plasmids: phalloidin (green), DAPI (blue), and Alexa Fluor 647-conjugated anti-Flag antibodies (red). Scale bar: 10 μm. c. SDS-PAGE of purified proteins. d. Western blot validation of protein expression. e. Bmp2 activity reporter assay: schematic of the luciferase reporter system (left), representative fluorescence images, and statistical analysis of firefly luciferase activity by in vivo imaging system <t>(IVIS)</t> (a.u.: arbitrary units) (right, n = 3 per group). f. qPCR analysis of Bmp2 signaling pathway-related mRNA levels in MC3T3-E1 cells treated with the Bmp2 or D-Bmp2 protein (n = 3 per group). g, h. Western blot (g) and quantification of Bmp2 pathway-related protein expression in treated MC3T3-E1 cells (h) (n = 3 per group). i. Schematic of the HA-coated ELISA plate and HA-binding affinity assay (n = 3 per group). j. Tissue-specific binding assay of Bmp2 or D-Bmp2: Mouse muscle slices (left, scale bar: 100 μm) and undecalcified femur slices (right, scale bar: 200 μm) stained with AF647-conjugated anti-Flag antibodies. The data are presented as the means ± standard deviations (SDs). One-way ANOVA was used for multiple comparisons. Significance levels: ns (not significant), ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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    MILabs vivo fluorescence distribution
    Alcohol consumption aggravates prostate inflammation and promotes M1 macrophage polarization in EAP mice. ( A ) Schematic of the experimental timeline for EAP induction and alcohol intervention. ( B ) The body weights of mice in the Control, Control+Alcohol, EAP, and EAP+Alcohol groups. ( C and D ) Response frequency to mechanical stimuli and the 50% mechanical withdrawal threshold. ( E ) Prostate inflammation score. ( F ) H&E staining of the anterior and dorsolateral prostate. Scale bars, 100 μm. ( G ) Percentage of CD45-positive staining in prostate immunohistochemistry. ( H ) Immunohistochemical staining of CD45 in prostate tissues. Scale bars, 100 μm. ( I-J ) Relative <t>fluorescence</t> intensity of CD86 and CD206 in prostate tissue. ( K ) Immunofluorescence staining of CD86 and CD206 in prostate tissue. Scale bars, 50 μm. Data are presented as mean ± SD (n = 6 for B-K). *, p < 0.05; ***, p < 0.001.
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    Caliper Life Sciences fluorescence in vivo imaging system
    Characterization of M2-exo@HI. (a) Western blot of Tsg101, CD9, and Calnexin expressions in RAW264.7 and M2-exo. (b) Protein bands of RAW264.7, M2-exo, M2-exo@HI, and HI by SDS-PAGE. (c) <t>Fluorescence</t> microscopy images showing Hp-EGFP and IL-10-mCherry expression in 293T cells infected with lipo2000@pBudCE4.1 , lipo2000@HI, and M2-exo@HI respectively, after 24 h. (d,e) Representative TEM images and size distribution profiles of M2-exo and M2-exo@HI. (f) Particle number of M2-exo and M2-exo@HI by NTA measurement. (g) Zeta potentials of M2-exo and M2-exo@HI (n = 3). (h) Drug release profiles of M2-exo@HI at pH 6.5 and pH 7.4, respectively (n = 3). (i) Stability evaluation of M2-exo@HI in PBS at 4 °C by monitoring particle size over time (n = 3). Data are presented as mean ± SD.
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    Image Search Results


    Design and validation of bone-targeted Bmp2. a. Schematic representation of the Bmp2/D-Bmp2 plasmid constructs and the structure predicted by AlphaFold3. b. Immunofluorescence staining of HEK-293T cells transfected with the plasmids: phalloidin (green), DAPI (blue), and Alexa Fluor 647-conjugated anti-Flag antibodies (red). Scale bar: 10 μm. c. SDS-PAGE of purified proteins. d. Western blot validation of protein expression. e. Bmp2 activity reporter assay: schematic of the luciferase reporter system (left), representative fluorescence images, and statistical analysis of firefly luciferase activity by in vivo imaging system (IVIS) (a.u.: arbitrary units) (right, n = 3 per group). f. qPCR analysis of Bmp2 signaling pathway-related mRNA levels in MC3T3-E1 cells treated with the Bmp2 or D-Bmp2 protein (n = 3 per group). g, h. Western blot (g) and quantification of Bmp2 pathway-related protein expression in treated MC3T3-E1 cells (h) (n = 3 per group). i. Schematic of the HA-coated ELISA plate and HA-binding affinity assay (n = 3 per group). j. Tissue-specific binding assay of Bmp2 or D-Bmp2: Mouse muscle slices (left, scale bar: 100 μm) and undecalcified femur slices (right, scale bar: 200 μm) stained with AF647-conjugated anti-Flag antibodies. The data are presented as the means ± standard deviations (SDs). One-way ANOVA was used for multiple comparisons. Significance levels: ns (not significant), ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

    Journal: Bioactive Materials

    Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification

    doi: 10.1016/j.bioactmat.2026.02.050

    Figure Lengend Snippet: Design and validation of bone-targeted Bmp2. a. Schematic representation of the Bmp2/D-Bmp2 plasmid constructs and the structure predicted by AlphaFold3. b. Immunofluorescence staining of HEK-293T cells transfected with the plasmids: phalloidin (green), DAPI (blue), and Alexa Fluor 647-conjugated anti-Flag antibodies (red). Scale bar: 10 μm. c. SDS-PAGE of purified proteins. d. Western blot validation of protein expression. e. Bmp2 activity reporter assay: schematic of the luciferase reporter system (left), representative fluorescence images, and statistical analysis of firefly luciferase activity by in vivo imaging system (IVIS) (a.u.: arbitrary units) (right, n = 3 per group). f. qPCR analysis of Bmp2 signaling pathway-related mRNA levels in MC3T3-E1 cells treated with the Bmp2 or D-Bmp2 protein (n = 3 per group). g, h. Western blot (g) and quantification of Bmp2 pathway-related protein expression in treated MC3T3-E1 cells (h) (n = 3 per group). i. Schematic of the HA-coated ELISA plate and HA-binding affinity assay (n = 3 per group). j. Tissue-specific binding assay of Bmp2 or D-Bmp2: Mouse muscle slices (left, scale bar: 100 μm) and undecalcified femur slices (right, scale bar: 200 μm) stained with AF647-conjugated anti-Flag antibodies. The data are presented as the means ± standard deviations (SDs). One-way ANOVA was used for multiple comparisons. Significance levels: ns (not significant), ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

    Article Snippet: After 24 h, the medium was changed to contain either Bmp2 or D-Bmp2 in DMEM, and the cells were further cultured for another 24 h. After the supernatant was removed, D-luciferin potassium salt (Beyotime, China) was added, and the firefly fluorescence signal was recorded using an in vivo imaging system (IVIS) (PerkinElmer, USA).

    Techniques: Biomarker Discovery, Plasmid Preparation, Construct, Immunofluorescence, Staining, Transfection, SDS Page, Purification, Western Blot, Expressing, Activity Assay, Reporter Assay, Luciferase, Fluorescence, In Vivo Imaging, Enzyme-linked Immunosorbent Assay, Binding Assay

    Alcohol consumption aggravates prostate inflammation and promotes M1 macrophage polarization in EAP mice. ( A ) Schematic of the experimental timeline for EAP induction and alcohol intervention. ( B ) The body weights of mice in the Control, Control+Alcohol, EAP, and EAP+Alcohol groups. ( C and D ) Response frequency to mechanical stimuli and the 50% mechanical withdrawal threshold. ( E ) Prostate inflammation score. ( F ) H&E staining of the anterior and dorsolateral prostate. Scale bars, 100 μm. ( G ) Percentage of CD45-positive staining in prostate immunohistochemistry. ( H ) Immunohistochemical staining of CD45 in prostate tissues. Scale bars, 100 μm. ( I-J ) Relative fluorescence intensity of CD86 and CD206 in prostate tissue. ( K ) Immunofluorescence staining of CD86 and CD206 in prostate tissue. Scale bars, 50 μm. Data are presented as mean ± SD (n = 6 for B-K). *, p < 0.05; ***, p < 0.001.

    Journal: International Journal of Nanomedicine

    Article Title: Lactobacillus johnsonii -Derived Extracellular Vesicles Ameliorate Alcohol-Exacerbated Experimental Autoimmune Prostatitis by Inhibiting M1 Macrophage Polarization

    doi: 10.2147/IJN.S596237

    Figure Lengend Snippet: Alcohol consumption aggravates prostate inflammation and promotes M1 macrophage polarization in EAP mice. ( A ) Schematic of the experimental timeline for EAP induction and alcohol intervention. ( B ) The body weights of mice in the Control, Control+Alcohol, EAP, and EAP+Alcohol groups. ( C and D ) Response frequency to mechanical stimuli and the 50% mechanical withdrawal threshold. ( E ) Prostate inflammation score. ( F ) H&E staining of the anterior and dorsolateral prostate. Scale bars, 100 μm. ( G ) Percentage of CD45-positive staining in prostate immunohistochemistry. ( H ) Immunohistochemical staining of CD45 in prostate tissues. Scale bars, 100 μm. ( I-J ) Relative fluorescence intensity of CD86 and CD206 in prostate tissue. ( K ) Immunofluorescence staining of CD86 and CD206 in prostate tissue. Scale bars, 50 μm. Data are presented as mean ± SD (n = 6 for B-K). *, p < 0.05; ***, p < 0.001.

    Article Snippet: The in vivo fluorescence distribution was evaluated 12 hours post-injection using an imaging system from MILabs (Netherlands).

    Techniques: Control, Staining, Immunohistochemistry, Immunohistochemical staining, Fluorescence, Immunofluorescence

    The gut microbiota mediates the effect of alcohol on EAP mice. ( A and B ) Response frequency to mechanical stimuli and the 50% mechanical withdrawal threshold. ( C ) Prostate inflammation score. ( D ) H&E staining of the anterior and dorsolateral prostate. Scale bars, 100 μm. ( E ) Immunohistochemical staining of CD45 in prostate tissues. Scale bars, 100 μm. ( F ) Percentage of CD45-positive staining in prostate immunohistochemistry. ( G ) Immunofluorescence staining of CD86 and CD206 in prostate tissue. Scale bars, 50 μm. ( H ) Relative fluorescence intensity of CD86 and CD206 in prostate tissue. ( I ) Pro-inflammatory cytokine levels in serum. ( J ) Pro-inflammatory cytokine mRNA expression in prostate tissues. Data are presented as mean ± SD (n = 6 for A-I, n = 4 for J). *, p < 0.05; **, p < 0.01; ***, p < 0.001.

    Journal: International Journal of Nanomedicine

    Article Title: Lactobacillus johnsonii -Derived Extracellular Vesicles Ameliorate Alcohol-Exacerbated Experimental Autoimmune Prostatitis by Inhibiting M1 Macrophage Polarization

    doi: 10.2147/IJN.S596237

    Figure Lengend Snippet: The gut microbiota mediates the effect of alcohol on EAP mice. ( A and B ) Response frequency to mechanical stimuli and the 50% mechanical withdrawal threshold. ( C ) Prostate inflammation score. ( D ) H&E staining of the anterior and dorsolateral prostate. Scale bars, 100 μm. ( E ) Immunohistochemical staining of CD45 in prostate tissues. Scale bars, 100 μm. ( F ) Percentage of CD45-positive staining in prostate immunohistochemistry. ( G ) Immunofluorescence staining of CD86 and CD206 in prostate tissue. Scale bars, 50 μm. ( H ) Relative fluorescence intensity of CD86 and CD206 in prostate tissue. ( I ) Pro-inflammatory cytokine levels in serum. ( J ) Pro-inflammatory cytokine mRNA expression in prostate tissues. Data are presented as mean ± SD (n = 6 for A-I, n = 4 for J). *, p < 0.05; **, p < 0.01; ***, p < 0.001.

    Article Snippet: The in vivo fluorescence distribution was evaluated 12 hours post-injection using an imaging system from MILabs (Netherlands).

    Techniques: Staining, Immunohistochemical staining, Immunohistochemistry, Immunofluorescence, Fluorescence, Expressing

    Transplantation of L. john relieves prostate inflammation and pain in EAP mice. ( A and B ) Response frequency to mechanical stimuli and the 50% mechanical withdrawal threshold. ( C ) Prostate inflammation score. ( D ) H&E staining of the anterior and dorsolateral prostate. Scale bars, 100 μm. ( E ) Percentage of CD45-positive staining in prostate immunohistochemistry. ( F ) Immunohistochemical staining of CD45 in prostate tissues. Scale bars, 100 μm. ( G-H ) Relative fluorescence intensity of CD86 and CD206 in prostate tissue. ( I ) Immunofluorescence staining of CD86 and CD206 in prostate tissue. Scale bars, 50 μm. Data are presented as mean ± SD (n = 6 for A-I). *, p < 0.05; **, p < 0.01.

    Journal: International Journal of Nanomedicine

    Article Title: Lactobacillus johnsonii -Derived Extracellular Vesicles Ameliorate Alcohol-Exacerbated Experimental Autoimmune Prostatitis by Inhibiting M1 Macrophage Polarization

    doi: 10.2147/IJN.S596237

    Figure Lengend Snippet: Transplantation of L. john relieves prostate inflammation and pain in EAP mice. ( A and B ) Response frequency to mechanical stimuli and the 50% mechanical withdrawal threshold. ( C ) Prostate inflammation score. ( D ) H&E staining of the anterior and dorsolateral prostate. Scale bars, 100 μm. ( E ) Percentage of CD45-positive staining in prostate immunohistochemistry. ( F ) Immunohistochemical staining of CD45 in prostate tissues. Scale bars, 100 μm. ( G-H ) Relative fluorescence intensity of CD86 and CD206 in prostate tissue. ( I ) Immunofluorescence staining of CD86 and CD206 in prostate tissue. Scale bars, 50 μm. Data are presented as mean ± SD (n = 6 for A-I). *, p < 0.05; **, p < 0.01.

    Article Snippet: The in vivo fluorescence distribution was evaluated 12 hours post-injection using an imaging system from MILabs (Netherlands).

    Techniques: Transplantation Assay, Staining, Immunohistochemistry, Immunohistochemical staining, Fluorescence, Immunofluorescence

    LjEVs alleviate prostate inflammation and inhibit M1 macrophage polarization in EAP mice. ( A ) Transmission electron microscopy (TEM) image of LjEVs. Scale bars, 100 nm. ( B and C ) Size distribution and concentration of LjEVs analyzed by nano-flow cytometry (nFCM). ( D and E ) The biodistribution of EVs@ICG versus free ICG was assessed in vivo and ex vivo after administration via tail vein injection. ( F and G ) Response frequency to mechanical stimuli and the 50% mechanical withdrawal threshold. ( H ) Prostate inflammation score. ( I ) H&E staining of the anterior and dorsolateral prostate. Scale bars, 100 μm. ( J ) Immunohistochemical staining of CD45 in prostate tissues. Scale bars, 100 μm. ( K ) Percentage of CD45-positive staining in prostate immunohistochemistry. ( L ) Immunofluorescence staining of CD86 and CD206 in prostate tissue. Scale bars, 50 μm. ( M ) Relative fluorescence intensity of CD86 and CD206 in prostate tissue. ( N ) Pro-inflammatory cytokine levels in serum. ( O ) Pro-inflammatory cytokine mRNA expression in prostate tissues. Data are presented as mean ± SD (n = 6 for F-N, n = 4 for O). *, p < 0.05; **, p < 0.01; ***, p < 0.001.

    Journal: International Journal of Nanomedicine

    Article Title: Lactobacillus johnsonii -Derived Extracellular Vesicles Ameliorate Alcohol-Exacerbated Experimental Autoimmune Prostatitis by Inhibiting M1 Macrophage Polarization

    doi: 10.2147/IJN.S596237

    Figure Lengend Snippet: LjEVs alleviate prostate inflammation and inhibit M1 macrophage polarization in EAP mice. ( A ) Transmission electron microscopy (TEM) image of LjEVs. Scale bars, 100 nm. ( B and C ) Size distribution and concentration of LjEVs analyzed by nano-flow cytometry (nFCM). ( D and E ) The biodistribution of EVs@ICG versus free ICG was assessed in vivo and ex vivo after administration via tail vein injection. ( F and G ) Response frequency to mechanical stimuli and the 50% mechanical withdrawal threshold. ( H ) Prostate inflammation score. ( I ) H&E staining of the anterior and dorsolateral prostate. Scale bars, 100 μm. ( J ) Immunohistochemical staining of CD45 in prostate tissues. Scale bars, 100 μm. ( K ) Percentage of CD45-positive staining in prostate immunohistochemistry. ( L ) Immunofluorescence staining of CD86 and CD206 in prostate tissue. Scale bars, 50 μm. ( M ) Relative fluorescence intensity of CD86 and CD206 in prostate tissue. ( N ) Pro-inflammatory cytokine levels in serum. ( O ) Pro-inflammatory cytokine mRNA expression in prostate tissues. Data are presented as mean ± SD (n = 6 for F-N, n = 4 for O). *, p < 0.05; **, p < 0.01; ***, p < 0.001.

    Article Snippet: The in vivo fluorescence distribution was evaluated 12 hours post-injection using an imaging system from MILabs (Netherlands).

    Techniques: Transmission Assay, Electron Microscopy, Concentration Assay, Flow Cytometry, In Vivo, Ex Vivo, Injection, Staining, Immunohistochemical staining, Immunohistochemistry, Immunofluorescence, Fluorescence, Expressing

    Characterization of M2-exo@HI. (a) Western blot of Tsg101, CD9, and Calnexin expressions in RAW264.7 and M2-exo. (b) Protein bands of RAW264.7, M2-exo, M2-exo@HI, and HI by SDS-PAGE. (c) Fluorescence microscopy images showing Hp-EGFP and IL-10-mCherry expression in 293T cells infected with lipo2000@pBudCE4.1 , lipo2000@HI, and M2-exo@HI respectively, after 24 h. (d,e) Representative TEM images and size distribution profiles of M2-exo and M2-exo@HI. (f) Particle number of M2-exo and M2-exo@HI by NTA measurement. (g) Zeta potentials of M2-exo and M2-exo@HI (n = 3). (h) Drug release profiles of M2-exo@HI at pH 6.5 and pH 7.4, respectively (n = 3). (i) Stability evaluation of M2-exo@HI in PBS at 4 °C by monitoring particle size over time (n = 3). Data are presented as mean ± SD.

    Journal: Bioactive Materials

    Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage

    doi: 10.1016/j.bioactmat.2026.01.047

    Figure Lengend Snippet: Characterization of M2-exo@HI. (a) Western blot of Tsg101, CD9, and Calnexin expressions in RAW264.7 and M2-exo. (b) Protein bands of RAW264.7, M2-exo, M2-exo@HI, and HI by SDS-PAGE. (c) Fluorescence microscopy images showing Hp-EGFP and IL-10-mCherry expression in 293T cells infected with lipo2000@pBudCE4.1 , lipo2000@HI, and M2-exo@HI respectively, after 24 h. (d,e) Representative TEM images and size distribution profiles of M2-exo and M2-exo@HI. (f) Particle number of M2-exo and M2-exo@HI by NTA measurement. (g) Zeta potentials of M2-exo and M2-exo@HI (n = 3). (h) Drug release profiles of M2-exo@HI at pH 6.5 and pH 7.4, respectively (n = 3). (i) Stability evaluation of M2-exo@HI in PBS at 4 °C by monitoring particle size over time (n = 3). Data are presented as mean ± SD.

    Article Snippet: Then mice were anesthetized with 5 % isoflurane and imaged using near-infrared fluorescence in vivo imaging system (IVIS, Caliper Life Sciences, USA) at predetermined time intervals (1 min, 5 min, 10 min, 15min, 2 h, 6 h, 12 h, 24 h).

    Techniques: Western Blot, SDS Page, Fluorescence, Microscopy, Expressing, Infection

    Validation of M2-exo targeting, Hp/IL-10 transfection expression, and Hp/Hb binding. (a) Fluorescence imaging showing cellular uptake of ICG and M2-exo@ICG by M1 microglia. (b,c) Flow cytometry and corresponding quantification of RhB and M2-exo@RhB internalized by M1 microglia (n = 3). (d,e) Schematic illustration and quantitative analysis of the in vitro phagocytosis-release kinetics of M2-exo@RhB in BV2 under ICH-mimicking stimulation (n = 6). (f) Fluorescence images showing Hp and IL-10 expression in M1 microglia treated with M2-exo@HI for 12, 24, 48, 72 h. (g) Mean fluorescence intensity (MFI) quantification of Hp and IL-10 expression (n = 3). (h,i) ELISA measurements of secreted Hp and IL-10 protein levels (n = 3). (j,k) qPCR analysis of relative Hp and IL-10 mRNA expression (n = 3). (l) Western blot detection of Hp and IL-10 protein expression. (m) Densitometric quantification of Hp and IL-10 protein levels from Western blot (n = 3). (n) Co-immunoprecipitation assay confirming the formation of Hp-Hb complex. Data are presented as mean ± SD. Statistical significance was calculated by unpaired Student's t -test (c and e), and one-way ANOVA with Tukey's multiple comparisons test (g-k and m).

    Journal: Bioactive Materials

    Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage

    doi: 10.1016/j.bioactmat.2026.01.047

    Figure Lengend Snippet: Validation of M2-exo targeting, Hp/IL-10 transfection expression, and Hp/Hb binding. (a) Fluorescence imaging showing cellular uptake of ICG and M2-exo@ICG by M1 microglia. (b,c) Flow cytometry and corresponding quantification of RhB and M2-exo@RhB internalized by M1 microglia (n = 3). (d,e) Schematic illustration and quantitative analysis of the in vitro phagocytosis-release kinetics of M2-exo@RhB in BV2 under ICH-mimicking stimulation (n = 6). (f) Fluorescence images showing Hp and IL-10 expression in M1 microglia treated with M2-exo@HI for 12, 24, 48, 72 h. (g) Mean fluorescence intensity (MFI) quantification of Hp and IL-10 expression (n = 3). (h,i) ELISA measurements of secreted Hp and IL-10 protein levels (n = 3). (j,k) qPCR analysis of relative Hp and IL-10 mRNA expression (n = 3). (l) Western blot detection of Hp and IL-10 protein expression. (m) Densitometric quantification of Hp and IL-10 protein levels from Western blot (n = 3). (n) Co-immunoprecipitation assay confirming the formation of Hp-Hb complex. Data are presented as mean ± SD. Statistical significance was calculated by unpaired Student's t -test (c and e), and one-way ANOVA with Tukey's multiple comparisons test (g-k and m).

    Article Snippet: Then mice were anesthetized with 5 % isoflurane and imaged using near-infrared fluorescence in vivo imaging system (IVIS, Caliper Life Sciences, USA) at predetermined time intervals (1 min, 5 min, 10 min, 15min, 2 h, 6 h, 12 h, 24 h).

    Techniques: Biomarker Discovery, Transfection, Expressing, Binding Assay, Fluorescence, Imaging, Flow Cytometry, In Vitro, Enzyme-linked Immunosorbent Assay, Western Blot, Co-Immunoprecipitation Assay

    M2-exo@HI promotes in vitro microglia polarization, BBB repair and neuroprotection. (a) Flow cytometry analysis of M1-type (CD86 + ) and M2-type microglia (CD163 + ) following treatment with different formulations. (b,c) Percentages of CD86 + and CD163 + microglia populations (n = 3). (d–g) The cytokine levels of IL-10, TGF-β, TNF-α, and IL-1β in treated microglia (n = 3). (h) Fluorescence microscopy images showing erythrophagocytosis by microglia across treatment groups. (i) Schematic of the in vitro BBB model assessing FITC-dextran permeability using a transwell assay. (j) Quantitative analysis of FITC-dextran penetration (n = 7). (k) Flow cytometry analysis of neuronal apoptosis across treatments (n = 3). (l) Quantitative analysis of neuronal apoptosis (n = 3). Data are presented as mean ± SD. Statistical significance was tested by one-way ANOVA with Tukey's multiple comparisons test.

    Journal: Bioactive Materials

    Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage

    doi: 10.1016/j.bioactmat.2026.01.047

    Figure Lengend Snippet: M2-exo@HI promotes in vitro microglia polarization, BBB repair and neuroprotection. (a) Flow cytometry analysis of M1-type (CD86 + ) and M2-type microglia (CD163 + ) following treatment with different formulations. (b,c) Percentages of CD86 + and CD163 + microglia populations (n = 3). (d–g) The cytokine levels of IL-10, TGF-β, TNF-α, and IL-1β in treated microglia (n = 3). (h) Fluorescence microscopy images showing erythrophagocytosis by microglia across treatment groups. (i) Schematic of the in vitro BBB model assessing FITC-dextran permeability using a transwell assay. (j) Quantitative analysis of FITC-dextran penetration (n = 7). (k) Flow cytometry analysis of neuronal apoptosis across treatments (n = 3). (l) Quantitative analysis of neuronal apoptosis (n = 3). Data are presented as mean ± SD. Statistical significance was tested by one-way ANOVA with Tukey's multiple comparisons test.

    Article Snippet: Then mice were anesthetized with 5 % isoflurane and imaged using near-infrared fluorescence in vivo imaging system (IVIS, Caliper Life Sciences, USA) at predetermined time intervals (1 min, 5 min, 10 min, 15min, 2 h, 6 h, 12 h, 24 h).

    Techniques: In Vitro, Flow Cytometry, Fluorescence, Microscopy, Permeability, Transwell Assay

    Targeted delivery and therapeutic gene expression of M2-exo@HI in hemorrhagic brain. (a) In vivo near-infrared fluorescence imaging showing ICG and M2-exo@ICG in mouse brains at various time points post-injection. (b) Average radiation efficiency of ICG in different treatment groups (n = 3). (c) Ex vivo fluorescence imaging of major organs harvested 24 h post-injection. (d) Average radiation efficiency of ICG in different in mouse tissues (n = 3). (e) Time-dependent accumulation of M2-exo@ICG at hematoma regions. (f) Immunofluorescence staining showing co-localization of Hp/IL-10 with astrocytes, microglia, and endothelial cells. (g) Temporal expression profiles of Hp and IL-10 in brain tissues. (h,i) ELISA quantification of Hp and IL-10 protein levels in brain homogenates (n = 3). Data are presented as mean ± SD. Statistical significance was calculated by unpaired Student's t -test (b,d), and one-way ANOVA with Tukey's multiple comparisons test (h,i).

    Journal: Bioactive Materials

    Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage

    doi: 10.1016/j.bioactmat.2026.01.047

    Figure Lengend Snippet: Targeted delivery and therapeutic gene expression of M2-exo@HI in hemorrhagic brain. (a) In vivo near-infrared fluorescence imaging showing ICG and M2-exo@ICG in mouse brains at various time points post-injection. (b) Average radiation efficiency of ICG in different treatment groups (n = 3). (c) Ex vivo fluorescence imaging of major organs harvested 24 h post-injection. (d) Average radiation efficiency of ICG in different in mouse tissues (n = 3). (e) Time-dependent accumulation of M2-exo@ICG at hematoma regions. (f) Immunofluorescence staining showing co-localization of Hp/IL-10 with astrocytes, microglia, and endothelial cells. (g) Temporal expression profiles of Hp and IL-10 in brain tissues. (h,i) ELISA quantification of Hp and IL-10 protein levels in brain homogenates (n = 3). Data are presented as mean ± SD. Statistical significance was calculated by unpaired Student's t -test (b,d), and one-way ANOVA with Tukey's multiple comparisons test (h,i).

    Article Snippet: Then mice were anesthetized with 5 % isoflurane and imaged using near-infrared fluorescence in vivo imaging system (IVIS, Caliper Life Sciences, USA) at predetermined time intervals (1 min, 5 min, 10 min, 15min, 2 h, 6 h, 12 h, 24 h).

    Techniques: Gene Expression, In Vivo, Fluorescence, Imaging, Injection, Ex Vivo, Immunofluorescence, Staining, Expressing, Enzyme-linked Immunosorbent Assay